Whole-ganglion imaging of voltage in the medicinal leech using a double-sided microscope
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A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensiti...
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Central pattern generators (CPGs) control both swimming and crawling in the medicinal leech. To investigate whether the neurons comprising these two CPGs are dedicated or multifunctional, we used voltage-sensitive dye imaging to record from approximately 80% of the approximately 400 neurons in a segmental ganglion. By eliciting swimming and crawling in the same preparation, we were able to iden...
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Voltage-dependent Na and Ca inward currents underlying the action potential in cultured embryonic ganglion cells of the leech Hirudo medicinalis have been investigated using the gigaseal whole-cell current or voltage-clamp technique. Dissociated ganglion cells were isolated from 7to 14-day-old embryos, and maintained in primary culture for up to 5 days. More than 95 % of the cultured cells had ...
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تاریخ انتشار 2017